Mhat3f cells

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WebbIndeed, in To overcome problems caused by limited transfection effi- the study that provided the data for the bioinformatic analysis ciency, mhAT3F cells were stably transfected with expression (21), Landgraf et al. reported failing to detect mature miR-124 vectors for miRNAs shown to target Mct1 (miR-29a, miR-29b, in mouse or human islets … Webb31 dec. 1996 · The glucose-induced GLUT2 mRNA accumulation was decreased by dibutyryl-cAMP both in mhAT3F cells and in primary hepatocytes. A putative cAMP-responsive element (CRE) is localized at the -1074/-1068 bp region of the promoter.

(PDF) miR-29a and miR-29b Contribute to Pancreatic -Cell-Specific ...

Webb8 mars 1996 · The mhAT3F cells were incubated for 24 h in the medium containing 20 nM insulin, 1 μM triiodothyronine, 1 μM dexamethasone, and 10 mM lactate and, after the … WebbmhAT3F or MIN6 cells were detached with trypsin and suspended in binding buffer (D-PBS 0.15% BSA). Cell suspensions were incubated for 1 h with anti-hGLUT2 … calonges 47

(PDF) A Karyopherin α2 Nuclear Transport Pathway is Regulated …

WebbWhen mhAT3F cells were cultured for 24 h in the presence of increasing glucose concentrations (0, 5, and 17 mmol/liter), an accumulation of Glut2 mRNA was observed (Fig. 1 ). Furthermore, glycogen accumulated in mhAT3F cells with a dose-response … Webb2 okt. 1998 · When mhAT3F cells were cultured for 24 h in the presence of increasing glucose concentrations (0, 5, and 17 mmol/liter), an accumulation of Glut2mRNA was … Webb1 mars 1997 · This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.privacy notice and cookie policy. cocotec in werl audia6 avant

An Unusual High-K m Hexokinase Is Expressed in the mhAT3F …

The large intracytoplasmic loop of the glucose transporter GLUT2 …

Webb1 feb. 1994 · Hepatocyte-like mhAT3F cells have been derived from the hepatoma of a transgenic mouse expressing the SV40 large T antigen under the control of the … coco test serverWebb29 maj 1995 · The genomic DNA of infected mhAT3F cells was extracted and used for PCR amplication using the same primers as those used for RT-PCR. Detection of the specific amplified fragment proved the integration of the retroviral sequences into the genome of infected cells. cocotein ingredients

"Webba GLUT2 expressing hepatoma cell line (mhAT3F) and into primarycultured rathepatocytes, and subsequently incubated at low and high glucose concentrations. Glucose stimulates gene transcription in a manner similar to that observed for the endogenous GLUT2 mRNA in both cell types; the ﬁ1308 to " - Mhat3f cells

Mhat3f cells

WebbmhAT3F cells MIN6 cells HEK293 cells Not stated Pullen et al. miRNA-939 SLC34A2 Gastric cancer cell lines and tissue 5-Fluorouacil Zhang et al. miRNA-369-3p SLC35F5 NSCLC cells ... Webb22 jan. 1999 · MhAT3F is a well differentiated mouse hepatocyte-like cell line ( 26, 32) that has a protein secretion profile very similar to primary hepatocytes. Cells were pretreated for 15 min with the MTP inhibitor prior to pulse labeling with l - [ 35 S]methionine for 30 min. After a 120-min chase, secreted apoB were immunoprecipitated.

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WebbGLUT2 mRNA amounts in mhAT3F cells expressing the GFPGLUT2 loop were always statistically lower than GLUT2 mRNA amounts in wild-type cells (P<0.01; n=4). Source … WebbAcute liver failure (ALF) is associated with massive hepatocyte cell death and high mortality rates. Therapeutic approaches targeting hepatocyte injury in ALF are hampered by the activation of distinct stimulus-dependent pathways, mechanism of cell death and a limited therapeutic window.

WebbThe mhAT3F cells were incubated for 24 h in the medium containing 20 nM insulin, 1 μM triiodothyronine, 1 μM dexamethasone, and 10 mM lactate and, after the … WebbMorphology of NIH3T3 cells during exposure to GF-AFC reagent. Panel A shows a field of cells photographed immediately after additional of the GF-AFC reagent. Panel B shows …

Webb5 okt. 2015 · This is not seen in the yeast cells in the two-hybrid assays possibly because additional factor(s) are necessary as mediators or cofactors for this connection. The … Webb1 jan. 1994 · This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Webbhexokinase activity is present in mhAT3F cells. This might explain why the effects of glucose on gene expres-sion were not maximal at a glucose concentration of 5 …

WebbCell Culture Human hepatoblastoma (HepG2) cells were routinely maintained in Williams’s medium (Eurobio, France) supplemented with 10% fetal calf serum and 1% streptomycin at 37°C. Only conﬂuent cells were used in the study. The well-differentiated mhAT3F hepatoma cell line was derived from the tumorous liver of a transgenic mouse cocotechWebb14 mars 2024 · the mhAT3F cells, immortalized, highly differentiated hepatocytes, maintained their epithelial morphology and underwent dramatic alterations of adhesion, leading to partial or complete detachment from a culture plate, followed by … coco tan of keller williams realtyWebb19 dec. 2007 · Transforming growth factor β (TGF-β) has a strong impact on liver development and physiopathology, exercised through its pleiotropic effects on growth, differentiation, survival, and migration. When exposed to TGF-β, the mhAT3F cells, immortalized, highly differentiated hepatocytes, maintained their epithelial morphology … coco teaser trailerWebb19 dec. 2007 · The mhAT3F undergo a cycle of contraction and respreading under the influence of TGF-β. Cells were grown on plastic dishes, treated with TGF-β1 (5 ng/ml), and observed by time-lapse microscopy for 96 h. Representative images of a low-power field and threefold enlargement of one cluster (indicated by an arrow in the top panel). coco technologyWebb23 okt. 2003 · In mhAT3F hepatoma cells, GFP-karyopherin α2 accumulated massively in the cytoplasm within minutes of glucose extracellular addition and returned to the … coco tchat bloquéWebb1 jan. 1994 · Hepatocyte-like mhAT3F cells have been derived from the hepatoma of a transgenic mouse expressing the SV40 large T antigen under the control of the … cocotamas crying compilation part 2WebbAmong them is the hepatocyte nuclear factor 3 (HNF3) family, composed of the HNF3 alpha, HNF3 beta, and HNF3 gamma proteins, which are expressed in the liver and have very similar fork head DNA binding domains. The regulatory regions of numerous hepatocyte-specific genes contain HNF3 binding sites. ca long term care bill