Nettet1. mar. 1988 · We therefore decided to study this question further, using the oocyte of Patella vulgata as a model system which may contain both MPF and CSF activities. In this paper, we show that MPF activation and amplification in Patella are pH-dependent but protein-synthesis-independent events that actually result in an increased … NettetJohn W. Newport* and Marc W. Kirschner Department of Biochemistry and Biophysics University of California San Francisco San Francisco, California 94143 Summary Maturation-promoting factor (MPF) is a partially pur- ified M-phase-specific activity that induces meiosis in frog oocytes and is detectable in mitotic lysates from cells of wide …
Canonical and Alternative Pathways in Cyclin-Dependent Kinase
Nettet18. nov. 1993 · CSF prevents ubiquitin-dependent degradation of mitotic cyclins and thus inactivation of the M phase-promoting factor (MPF) 3,4.Fertilization or parthenogenetic … NettetIn vertebrates, M phase-promoting factor (MPF), a universal G2/M regulator in eukaryotic cells, drives meiotic maturation of oocytes, while cytostatic factor (CSF) arrests mature oocytes at ... general provision tax treatment
Cytoplasmic control of nuclear behavior during meiotic maturation …
NettetRecent studies in other laboratories indicate that primary CSF is the c-mos proto-oncogene product. The effect of CSF appears to be primarily stabilization of maturation-promoting … Nettet26. jan. 2007 · The long-term stability of MPF is unique to eggs since in a mitotic metaphase, the APC/C would be active and cyclin B degraded. Although it is possible to exit CSF mediated MetII arrest by inhibiting the CDK1 component of MPF [ 21, 22 ], physiologically a sperm Ca 2+ signal induces loss of cyclin B rather than CDK1 … Nettet5. mai 1998 · Although CSF prevents the degradation of cyclin, the inactivation of CSF itself is not required for the proteolysis of the cyclin subunit of MPF and for the resulting exit from meiotic metaphase. In cytosolic extracts from MII arrested eggs of both amphibians and mammals, MPF is inactivated before CSF 13, 14. general provisions on ships\u0027 routeing