Phosphomotif antibodies

Author: rtmo

August undefined, 2024

WebPhosphoSitePlus Protein or Substrate Search Alternative Search Options Protein, Sequence, or Reference Search Protein, Sequence, or Reference Search: Protein Searches retrieve lists of proteins and their modification types based on protein name or ID, protein type, domain, cellular component, MW, and pI range. WebNov 11, 2013 · Here we have used phosphomotif antibodies and mass spectrometry (MS) to investigate the effects of MEK/PI3K dual inhibition during the period immediately preceding cell death. Upon treatment, melanoma cell lines responded by dramatically increasing phosphorylation on proteins containing a canonical DNA damage-response (DDR) motif, …

Using Phospho-Motif Antibodies to Determine Kinase Substrates

WebApr 1, 2009 · Phosphomotif antibody 5557 is phosphospecific and detects MAPK phosphorylation events.A, sequence of phosphopeptide library containing two … WebJan 9, 2014 · After 2D-DIGE analysis, changed protein spots were characterized for reactivity to ERK phosphomotif antibodies and identified by MS. Various types of phosphomotif … simply.io

Novel insulin-elicited phosphoproteins in adipocytes - ScienceDirect

WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and … WebSep 20, 2009 · Using a phosphoproteomic approach combining the steroid receptor fusion system, IMAC, 2D-DIGE and phosphomotif-specific antibodies, we detected 38 proteins … WebSep 29, 2014 · Atg9A bears a conserved canonical 14-3-3ζ binding phosphomotif. (A) HA-Atg9A was overexpressed in HEK-293 cells, and cells were treated with hypoxia for the indicated times. Immunoprecipitated HA-Atg9A was immunoblotted with an anti-14-3-3ζ phosphomotif antibody (pMotif) and for endogenous 14-3-3ζ. simply ioa 5k

Antiphospholipid syndrome - Symptoms and causes

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WebMay 13, 2015 · The ability of anti-mHttexon1 antibodies and antibody fragments to reduce the aggregation and associated neurotoxicity has proven to be strongly dependent on the targeted epitope . Specifically targeting the Htt sequence between 1 and 17, which has been shown to play a key role in aggregation and HD pathology, seems to be an obvious choice. WebPhosphoNET is an open-access, online resource developed by Kinexus Bioinformatics Corporation to foster the study of cell signalling systems to advance biomedical research in academia and industry. PhosphoNET is the worlds largest repository of known and predicted information on human phosphorylation sites, their evolutionary conservation, the ... simply invoke it with your regular userWebphosphomotif antibodies, indicating the pos-sibility of direct ERK substrates. From this screen, 38 candidate targets were identi-fied by mass spectrometry (MS), includ-ing 13 new substrates that were verified by in vitro ERK phosphorylation. Further stud-ies of one of them, the nucleoporin Nup50, revealed an unexpected role of ERK signal- simply ioa reviews

"WebJan 4, 2013 · These antibodies are commonly used in immunoprecipitation and/or immunoblotting protocols to determine kinase-induced substrate phosphorylation. This … " - Phosphomotif antibodies

Phosphomotif antibodies

WebJan 1, 2005 · The antibody reactive with the phosphomotif generated by Akt, referred to here as the PAS (phospho Akt substrate) antibody, was purchased from Cell Signaling Technology, Beverly, MA (catalog number 9611). Rabbit antisera against peptides from the insulin-elicited phosphoproteins were generated for us by BioSource International, … Web2024年4月の新製品リストです。

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Webantibodies, generally crosslinked to a resin, targeting a consensus phosphomotif or a specific phosphoresidue, such as phosphotyrosine (pTyr) 13,14. This last option is highly specific but is generally limited due to the cost of use. IAP can be less efficient in complex samples due to the high background WebPhosphosite-specific antibodies can be produced as polyclonals or monoclonals in different animal species, and each approach offers its own benefits and disadvantages. The validation of phosphosite-specific antibodies requires multiple techniques and tactics to demonstrate their specificity.

WebAntiphospholipid antibodies are antibodies directed against phosphorus-fat components of your cell membranes called phospholipids, certain blood proteins that bind with …

WebApr 11, 2024 · Ph2AX antibody was initially tested on cisplatin-treated and untreated iKras PDAC cells embedded in agarose, and an optimal dilution of 1:400 was determined for the detection of DNA damage cells by IHC staining. Primary and secondary OmniMap HRP-conjugated antibodies were used in 100 μl volumes, for 60 and 16 min, respectively. WebATM-substrate phosphomotif blots showed a single, low molecular weight feature which similarly decreased in cells when PI3K was inhibited (i.e., in cells treated with PI3Ki alone …

Webnonphosphorylatable Ser172Ala mutation abolished interaction with the antibody to the 14-3-3 binding motif, whereas a nonphosphorylatable Ser155Ala mutation abolished the interaction with the antibody to the MPK substrate motif, and mutation of either site alone did not affect the AICAR-induced phosphorylation of the other site (Fig. 3C).

WebProteins were extracted from each cell line and digested with trypsin. For phosphopeptide enrichment in serial immunoaffinity purifications, phosphomotif antibodies were used in the following order: PXpSP, pTP, RXRXXpS, and RXXpS where “X” represents any amino acid and “p” reflects the phosphorylation site. The samples were run in ... raytheon mawson lakesWebPhosphosite-specific antibodies can be produced as polyclonals or monoclonals in different animal species, and each approach offers its own benefits and disadvantages. The … raytheon mbe laserhttp://www.phosphonet.ca/default.aspx simply invoice softwareWebDownload scientific diagram Phosphomotif antibody 5557 is phosphospecific and detects MAPK phosphorylation events. A, sequence of phosphopeptide library containing two … raytheon massachusetts phoneWebDec 8, 2015 · Together, using a TNIK inhibitor and phosphomotif antibody, we identify endogenous substrates of TNIK in neurons, define consensus sequences for TNIK, and suggest signaling pathways by which... raytheon mbmmrWebJan 15, 2016 · To examine endogenous MFF phosphorylation, we developed a phosphospecific antibody to phospho-Ser 172 (P-Ser 172), which we validated for … raytheon massachusetts lowellWebAntibodies—A key reagent for this study is the antibody for the Akt phosphomotif RXRXXpS/T, where X is any amino acid. This antibody, which is referred as the PAS antibody (phospho Akt substrate), was purchased from Cell Signaling Technology, Beverly, MA (catalog num-ber 9611). It is an affinity-purified rabbit antibody that has been shown raytheon mbse